polysome profiling RNA seq experiments on extracts from METTL3-WT and METTL3-K177Q reconstituting cells
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE183016
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Given the overall steady-state mRNA abundance between these two groups displayed negligible differences, these data implied an increased translational efficiency in cells bearing K177Q mutation. Using sucrose gradient centrifugation, collected fractions were pulled into sub-polysome fraction and polysome fraction. Then, sub-polysome and polysome samples were subjected to RNA-seq.
创建时间：
2022-11-02
