S-adenosylmethionine treatment affects histone methylation in prostate cancer cells

S-adenosylmethionine represents a potent inhibitor of cancer cell proliferation, migration and invasion in vitro. The reason remains unclear. Here, we examined if treatment with exogenous SAM is capable of causing alterations in the methylation of the histone markers H3K4me3 and H3K27me3, which are both known to be important in the initiation and progression of prostate cancer. Overall design: PC-3 cells were treated with 200µmol SAM, a concentration known to cause anticancerogenic effects. The treatment was stopped after 120h. Three treated samples and controls (as well as input samples) were used to perform ChIP with antibodies against H3K4me3 and H3K27me3. Subsequently massive parallel sequencing was performed for all samples and controls. Exogenous treatment with SAM affects Histone methylation in prostate cancer cells.