Untitled Item
收藏DataCite Commons2022-01-08 更新2024-07-28 收录
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RNA-seq procedure: Raw sequence data have been deposited in the ArrayExpress database at EMBL-EBI (accession number E-MTAB-8988). Processing of raw sequence data was performed with fastp (Chen et al. 2018) using default parameters. The processed sequences were mapped against the transcriptome of Populus euphratica (GCF_000495115.1_PopEup_1.0_rna.fna.gz, https://www.ncbi.nlm.nih.gov/genome/13265) using Bowtie 2 (Langmead and Salzberg, 2012). Depending on the sample, 88.4 to 92.1 % of all filtered reads could be mapped to a gene model. Bowtie mapping files were summarized to count tables of the gene models in R (R Core Team 2018) (Supplemental Table S1). Identification of differentially expressed transcripts was conducted using the DESeq2 package (Love et al., 2014), using a generalized linear model with Benjamini and Hochberg correction to adjust p-values.
提供机构：
figshare
创建时间：
2021-11-30
