K<sub>app</sub> constants of PRO and selected mutants in an Ub-AMC hydrolysis assay far from saturating conditions.
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*Kapp is reported according to the equation V/[E] = kapp [S]. All experiments were performed three to five times. Data are expressed as the averages and standard deviations of these independent experiments. **significantly lower than PROwt (p<0.01) in the Mann-Whitney rank test using the five experiments for each enzyme.
创建时间：
2013-08-15
