16S ribosomal RNA sequencing of the V3-V4 region was performed by PCR. Sequencing library was generated and quality was assessed by Quant-iT PicoGreen (Invitrogen, USA). Then the library was sequenced
16S ribosomal RNA sequencing of the V3-V4 region was performed by PCR. Sequencing library was generated and quality was assessed by Quant-iT PicoGreen (Invitrogen, USA). Then the library was sequenced
The V3-V4 region of the 16S ribosomal RNA gene was targeted for amplicon sequencing using sequence-specific primers (341F: CCTAYGGGRBGCASCAG, 806R: GGACTACNNGGGTATCTAAT) with attached Illumina adapter