aThe total volume of PCR reaction was 25 µl and the PCR conditions were as follows: 50°C for 2 min, 95°C for 10 min, followed by 40 cycles of 95°C, 15 s for denaturation and 60°C, 1 min for annealing

NIAID Data Ecosystem20

Primer sequences used for mRNA quantification by real-time PCR.

基因表达定量

实时PCR

11β-HSD1, 11β-hydroxysteroid dehydrogenase type 1; 5αR1, 5α-reductase type 1; 5βR, 5β-reductase; GR, glucocorticoid receptor; C/EBPα, CCAAT/enhancer-binding protein α; C/EBPβ, CCAAT/enhancer-binding p

Figshare2015-12-02 更新10

qPCR primer sequences.

qPCR引物序列

基因表达定量

qPCR primer sequences.

NIAID Data Ecosystem30

Primer and Probes with optimized temperature conditions for real-time PCR.

定量PCR引物设计

分子生物学温度优化

Forward, sense primer; Reverse, antisense primer; Cy5, Cyanine 5; Phos, Phosphate; FL, fluorescein; A, annealing time; E, extension time.

Figshare2015-12-02 更新10