Mouse spleen LCM-ATACseq (B and T cell zones)
收藏NIAID Data Ecosystem2026-05-01 收录
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE237824
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资源简介：
In situ transposition followed by laser capture microdissection was applied to spleen mouse tissue sections to demonstrate feasibility of LCM-based spatial chromatin accessibility analysis of regions of interest. B220 and CD3e immunostained B and T cell zones were analyzed. Fresh frozen spleen tissue sections from C57BL/6J WT mice were tagmented in situ to assess chromatin accessibility. LCM was employed to collect a mini bulk of immunostained B and T cells. Two mini-bulk sizes were investigated: 100 single-captured nuclei or 10 larger captured-areas (patches) composed each of roughly 100 nuclei, for a total of ~1000 nuclei. Four technical replicates were collected at least per condition.
创建时间：
2023-12-01
