<b>Figure 2. UL12.5 is required for Phase I lytic gene expression during HSV-1 reactivation.</b><b> </b><b>UL12.5 is required for </b><b>Phase I </b><b>lytic gene expression during HSV</b><b>-1</b><b> reactivation</b>
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2B. Quantification of the relative viral genome (vDNA - <i>ICP8</i>) in latently infected (0 hours) and reactivated (60 hours) neurons infected with either KOS-SPA and KOS-UL98 measured by qPCR .<br>2C. Relative expression of lytic mRNAs and <i>LAT</i> in neurons latently infected (0 hours post reactivation) with either KOS-SPA and KOS-UL98 measured by RT-qPCR for <i>ICP27</i> (IE), <i>UL30</i> (E), <i>VP16</i> (L), and <i>g</i><i>C</i> (L).<br>2D. Relative expression of lytic mRNAs (<i>ICP27;UL30;VP16;gC</i>) at 18 hours post-reactivation (Phase I).<br>2E. Relative expression of lytic mRNAs (<i>ICP27;UL30;VP16;gC</i>) at 48 hours post-reactivation (Phase II).<br>2F. Relative expression of <i>ICP27</i> mRNA during Phase I reactivation in neurons latently infected with F/L or the rescue virus; F/LR.<br>2G. Relative expression of <i>ICP27</i> mRNA during Phase I reactivation in neurons latently infected with AN-1 or the wild-type strain KOS.
提供机构:
Cliffe, Anna; Flores, Matthew; Krakowiak, Patryk; Cuddy, Sean; Babnis, Aleksandra
创建时间:
2025-01-02



