In silico screening, biochemical characterization, and structural analysis of recombinant dimethylsulfide monooxygenases catalysing nicotinamide adenine dinucleotide:flavin mononucleotide oxidoreduction

Recombinant technology is the preferred method for the massive production of biocatalysts. However, overexpression of foreign proteins often results in inclusion body formation. In this study, a systematic in silico screening method was developed to screen for active and soluble biocatalyst. The success rate of producing soluble and active DmoA is 71%. The enzyme screening methodology established from this study can increase the success rate of producing soluble and functional enzymes while avoiding the laborious trial-and-error procedure. Interestingly, the DmoA exhibited oxidoreductase activity. This novel finding may allow DmoA to be used as an oxidoreductase biocatalyst for various industrial applications.