A novel transposon-based approach to analyze the 3D structure of the mouse genome in the cell nucleus.

The 3D organization of the genome has recently been considered to have essential impact on nuclear functions such as gene expression and epigenetic modifications. In this project, we develop a novel application of next-generation sequencing technology to analyze the 3D genome organization in the cell nucleus, in combination with a transposon-based genome engineering approach. Most DNA transposons have a tendency to jump around locally in the genome, referred to as “local hopping” tendency. By using next-generation sequencing technology, we globally identify the transposon insertion sites in a genome-wide manner, and investigate the correlation between the distribution of transposons and the spatial organization of the genome.