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Neuronal Methylome in Multiple Sclerosis

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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE119532
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DNA methylation profiling of NeuN+sorted neuronal nuclei from post-mortem brain tissue of Multiple Sclerosis (MS) patients (n=10) (MS) and non-neurological controls (n=7) (non-MS). Genomic DNA was subjected to conventional BS-treatment as well as oxidative BS (oxBS)-conversion using TrueMethylTM 96 kit of CEGXTM (Cambridge Epigenetix Limited) to allow for subsequent detection of hydroxymethylation (5hmC = BS - oxBS). Detection of DNA methylation differences (oxBS, BS and 5hmC) in NeuN+ sorted from white matter brain tissue of MS patients and non-neurological controls
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2019-06-21
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