High-throughput exploration of the network dependent on AKT1 in mouse ovarian granulosa cells.

We depleted Akt1 in murine primary granulosa cells and assessed the molecular consequences at the transcript level. We were able to corroborate the involvement of AKT1 in the regulation of metabolism, apoptosis, cell cycle or cytoskeleton dynamics in this ovarian cell type.  Overall design: Single Read 75 pb runs, on an Illumina NextSeq500 instrument, using NextSeq 500 High Output 75 cycles kits.