Activity-induced gene expression and long-range enhancer-promoter contacts in cohesin-deficient neurons (NexCre)

RNA-seq of total RNA from Rad21lox/lox;NexCre/+ and Rad21+/+;NexCre/+ dissociated cortical neurons at Day In Vitro (DIV10) without stimulation or after treatment with TTX/D-AP5 and 1h or 6h of KCl depolarization. Overall design: RNA-Seq was performed on total RNA from DIV10 cortical dissociated neurons. Neurons were left without stimulation, treated overnight with TTX/D-AP5 or treated overnight with TTX/D-AP5 and then depolarized for 1h or 6h with KCl. Three replicates of Rad21lox/lox;NexCre/+ and three replicates of Rad21+/+;NexCre/+ DIV10 cortical dissociated neurons in each condition were used.