BIOGRID CURATED DATA FOR PUBLICATION: Ndj1, a telomere-associated protein, regulates centrosome separation in budding yeast meiosis.

Protein-Protein, Genetic, and Chemical Interactions for Li P (2015):Ndj1, a telomere-associated protein, regulates centrosome separation in budding yeast meiosis. curated by BioGRID (https://thebiogrid.org); ABSTRACT: Yeast centrosomes (called spindle pole bodies [SPBs]) remain cohesive for hours during meiotic G2 when recombination takes place. In contrast, SPBs separate within minutes after duplication in vegetative cells. We report here that Ndj1, a previously known meiosis-specific telomere-associated protein, is required for protecting SPB cohesion. Ndj1 localizes to the SPB but dissociates from it ∼16 min before SPB separation. Without Ndj1, meiotic SPBs lost cohesion prematurely, whereas overproduction of Ndj1 delayed SPB separation. When produced ectopically in vegetative cells, Ndj1 caused SPB separation defects and cell lethality. Localization of Ndj1 to the SPB depended on the SUN domain protein Mps3, and removal of the N terminus of Mps3 allowed SPB separation and suppressed the lethality of NDJ1-expressing vegetative cells. Finally, we show that Ndj1 forms oligomeric complexes with Mps3, and that the Polo-like kinase Cdc5 regulates Ndj1 protein stability and SPB separation. These findings reveal the underlying mechanism that coordinates yeast centrosome dynamics with meiotic telomere movement and cell cycle progression.

蛋白质-蛋白质、遗传及化学相互作用数据集 Li P (2015)：Ndj1，一种与端粒相关的蛋白，调控芽殖酵母减数分裂中的中心体分离。由 BioGRID（https://thebiogrid.org）整理；摘要：酵母中心体（称为纺锤体极体[SPBs]）在减数分裂G2期重组发生期间保持凝聚力数小时。然而，在营养细胞中，SPBs在复制后几分钟内即分离。本研究报告指出，Ndj1，一种先前已知的减数分裂特异性端粒相关蛋白，对于保护SPB的凝聚力至关重要。Ndj1定位于SPB，但在SPB分离前约16分钟与之解离。缺乏Ndj1时，减数分裂SPB过早失去凝聚力；而Ndj1过度表达则延迟SPB分离。当在营养细胞中异位表达时，Ndj1会导致SPB分离缺陷和细胞死亡。Ndj1定位至SPB依赖于SUN结构域蛋白Mps3，Mps3的N端去除允许SPB分离并抑制NDJ1表达营养细胞的致死性。最终，我们揭示了Ndj1与Mps3形成寡聚复合物，以及 Polo样激酶Cdc5调节Ndj1蛋白稳定性和SPB分离的现象。这些发现揭示了酵母中心体动态与减数分裂端粒运动及细胞周期进程协调的潜在机制。