Axon guidance signal ensures neurite growth pace while sensitizing repulsive cues through induction of a dual function facilitator

Purpose: profiling the differential transcripts usage in wild type and Plxnd1 knockout condition of brain Methods: transcriptome analysis by the RNA-seq through the mRNA pull-down Results: We performed RNA-seq analysis from neonatal mouse striatum and found that the repulsive Semaphorin 3E (Sema3E)-Plexin-D1 guidance signaling transcriptionally upregulates Mtss1 in striatonigral projecting neurons of basal ganglia circuitry Conclusions: our findings demonstrate a molecular mechanism in which repulsive guidance cues activate an autoregulatory program enabling growing axons to perform both avoiding repellants and continuing axonal extension in the navigation to their target Overall design: Profiling the differential transcripts usage in wild type and Plxnd1 knockout condition of brain