­Itaconate-producing neutrophils regulate local and systemic inflammation following trauma

Itaconate-producing neutrophils regulate local and systemic inflammation following trauma 10-week old mice were subjected to burn to the back in combination with a full tenotomy of the Achille's tendon. Bone marrow, mononucleated cells from the blood and the tendon injury site (single cell suspension generated with enzymatic digetsion) were isolated Unsorted cells isolated from the bone marrow, blood and HO injury site 3 days post burn/tenotomy surgery