Dual RNA-seq of A549 cells infected with Nocardia at different time point

To fully understand the pathogenic mechanism of Nocardia and the adaptive response mechanism of the host response to Nocardia infection, the dual RNA-seq approach was applied in this study. To characterize the interactions of N. farcinica with lung epithelial cells, an in vitro infection model was built using human alveolar epithelial cells (A549). N. farcinica was used to infect A549 cells for 1, 3, and 6 h at an MOI of 10. Dual RNA-seq was used to determine genome-scale expression events in both hosts and bacteria at 1, 3, and 6 hours post-infection.