Supporting material for: Paediatric Leukaemia DNA methylation profiling using MBD enrichment and SOLiD Sequencing on archival bone marrow smears.

Acute Lymphoblastic Leukaemia(ALL) is the most common cancer in children. Over the past four decades, research has advanced the treatment of this cancer from less than 60% chance of survival to over 85% today. The causal molecular mechanisms remain unclear.
We performed SOLiD sequencing on Methyl-Binding Domain(MBD) enriched fractions of genomic DNA from eight paediatric ALL patients using archived bone marrow smear microscope slides. Primary tumour and remission bone marrow sample was analysed. Four patients relapsed and the relapsed tumour was analysed. Input and MBD-enriched DNA from each sample was sequenced, aligned to the hg19 reference genome and analysed for enrichment peaks using MACS and HOMER. In total, 3.67Gb were sequenced, 2.74Gb were aligned to the reference genome (average 74.66% alignment efficiency).
Our dataset represents one of the first SOLiD MBD-Seq studies performed on paediatric ALL and is the first to utilise archival bone marrow smears.