Transcriptional Profile of Microglia in Neurodegeneration

Purpose: RNASeq was used to identify transcriptional signature responsible for the metabolic reprogramming phenotype of microglia in neurodegenerative disease Result: We identified Ppargc1a/Cebpb signaling pathway as a critical axis that controls microglial glycolytic transition during neurodegeneration Overall design: Microglia from the midbrain region of animals with microglial Ppargc1a deficiency or controls were subjected to MPTP injections to induce neurodegeneration or treated with PBS as control. Total RNA from 3 microglia samples from each genotype/condition were isolated and used to prepare RNASeq library and analysis