MR1-restricted T-cell clonotypes are associated with 'resistance' to Mycobacterium Tuberculosis infection - TRA/D immunoSEQ

Summary

Number of files: 39

Data format: tsv

Data type: TCRA/D immunoSEQ (Adaptive Biotechnologies)

immunoSEQ version: v2

Sample type: peripheral blood mononuclear cells (PBMCs)

Genomic DNA extraction protocol: QIAGEN DNeasy Blood and Tissue kit

Sample description

Peripheral blood samples were collected from participants enrolled in a longitudinal cohort study based in Uganda. Donors were classified as either "resistors" (RSTRs, n = 19, defined as concordantly negative for tuberculin skin test and IFNg release assay despite high environmental exposure) or latently infected with M. Tuberculosis (LTBI, n = 20, concordantly as longitudinally positive for tuberculin skin test and IFNg release assay). Genomic DNA (gDNA) was extracted from cryopreserved PBMCs without stimulation or enrichment of T-cells using the Quiagen DNeasy Blood and Tissue kit. Protocol can be found at: https://www.qiagen.com/us/products/discovery-and-translational-research/dna-rna-purification/dna-purification/genomic-dna/dneasy-blood-and-tissue-kit