Genotype-Specific Root Morphology and Metabolic Traits Shape Bacterial Communities and Tolerance to Fusarium Root Rot in Wheat

This dataset comprises 16S rRNA gene amplicon sequences obtained from the rhizosphere and root endosphere of four wheat (Triticum aestivum) varieties—Concret, Mutic, Oregrain, and Pilier—grown under controlled conditions. These varieties display contrasting levels of tolerance to Fusarium graminearum, the causal agent of Fusarium root rot (FRR). The sequencing effort was conducted as part of a doctoral study at the Université de Lorraine (France) aimed at characterising the influence of wheat genotype and FRR tolerance on root-associated bacterial community structure and diversity. DNA was extracted from rhizosphere soil and surface-sterilised root samples and subjected to 16S rRNA gene amplicon sequencing, targeting the V5–V7 regions. Library preparation followed standard protocols for Illumina MiSeq paired-end sequencing (2×250 bp) and was performed by Genoscreen (France). A total of 40 samples (five biological replicates per genotype and compartment) were sequenced. For each sample, two FASTQ files were generated (R1 and R2) corresponding to forward and reverse reads; primer sequences were removed from the raw reads. These sequence data can be used for downstream analyses of microbial community diversity and composition (e.g., taxonomic assignment, alpha and beta diversity, community profiling). Complementary metabolomics data, generated by untargeted Orbitrap LC-MS analysis in positive and negative ion modes, include 879 metabolic features that can be used to explore genotype-specific metabolic–microbiome relationships.