Genotype-Specific Root Morphology and Metabolic Traits Shape Bacterial Communities and Tolerance to Fusarium Root Rot in Wheat

This dataset comprises 16S rRNA gene amplicon sequences obtained from the rhizosphere and root endosphere of four wheat (Triticum aestivum) varieties—Concret, Mutic, Oregrain, and Pilier—grown under controlled conditions. These varieties display contrasting levels of tolerance to Fusarium graminearum, the causal agent of Fusarium root rot (FRR). The sequencing effort was conducted as part of a doctoral study at the Université de Lorraine (France) aimed at characterising the influence of wheat genotype and FRR tolerance on root-associated bacterial community structure and diversity. DNA was extracted from rhizosphere soil and surface-sterilised root samples and subjected to 16S rRNA gene amplicon sequencing, targeting the V5–V7 regions. Library preparation followed standard protocols for Illumina MiSeq paired-end sequencing (2×250 bp) and was performed by Genoscreen (France). A total of 40 samples (five biological replicates per genotype and compartment) were sequenced. For each sample, two FASTQ files were generated (R1 and R2) corresponding to forward and reverse reads; primer sequences were removed from the raw reads. These sequence data can be used for downstream analyses of microbial community diversity and composition (e.g., taxonomic assignment, alpha and beta diversity, community profiling). Complementary metabolomics data, generated by untargeted Orbitrap LC-MS analysis in positive and negative ion modes, include 879 metabolic features that can be used to explore genotype-specific metabolic–microbiome relationships.本数据集包含从四个普通小麦（Triticum aestivum）品种——Concret、Mutic、Oregrain和Pilier——的根际（rhizosphere）与根内空间（root endosphere）获取的16S rRNA基因扩增子序列，所有供试材料均在可控环境条件下培育。上述小麦品种对禾谷镰孢菌（Fusarium graminearum，引发镰孢菌根腐病（FRR））的耐受性水平存在显著差异。本测序工作是法国洛林大学（Université de Lorraine）一项博士研究的组成部分，旨在解析小麦基因型与镰孢菌根腐病耐受性对根系相关细菌群落结构与多样性的影响。研究人员从根际土壤与经表面灭菌的根系样本中提取基因组DNA，针对16S rRNA基因的V5-V7高变区域开展扩增子测序。文库制备遵循Illumina MiSeq双端测序（2×250 bp）的标准流程，由法国Genoscreen公司完成。本数据集共包含40个测序样本，每个基因型与群落分区各设置5个生物学重复。每个样本生成两个FASTQ格式文件（R1与R2），分别对应正向与反向测序读段，原始测序读段已完成引物序列切除。上述序列数据可用于微生物群落多样性与组成的下游分析，例如物种分类注释、α多样性与β多样性分析、群落谱图构建。此外还配套了非靶向Orbitrap液相色谱-质谱（LC-MS）分析获取的代谢组学数据，涵盖正负离子模式下的879个代谢特征，可用于探索基因型特异性的代谢-微生物组关联关系。

搜集汇总

背景与挑战

背景概述

该数据集包含四个不同耐受镰刀菌根腐病（FRR）的小麦品种的根际和根内细菌16S rRNA基因序列，以及互补的代谢组学数据，旨在研究小麦基因型特异性根形态和代谢特征如何塑造细菌群落并影响对FRR的耐受性。数据来自法国洛林大学的博士项目，采用Illumina MiSeq测序技术，共40个样本，可用于微生物群落多样性、组成分析及代谢-微生物组关系探索。