Solubility and secondary structure of binary alternating peptides.

1 The secondary structure was estimated from the CD spectrum of the precipitate derived from a 0.2 mg/ml solution of peptide in 50 mM phosphate buffer pH 8. When the precipitate was transferred to the pH 4 buffer, the spectra were essentially the same. 2 The amount of the peptide in the supernatant and precipitate fractions was determined by hydrolysis (see Materials and Methods). 3 The CD spectra of samples indicated had no sufficient precipitate to be measured. 4 The quantitation of this sample was prevented by an interfering compound (HPLC). Solubility and secondary structure of binary alternating peptides.