Gossypium arboreum transcriptome assembly using 454 sequencing

The sequencing was performed on 454/Roche FLX technology using normalized cDNA libraries prepared from young leaves, roots, bolls, ovules, and fibers of G. arboreum L. Reads from two independent sequencing samples were combined to assemble contigs. The combined sequencing analysis using 1,699,776 reads from A-genome resulted in 89,588 contigs for A-genome. The majority of contigs (~70%) ranged from 200 to 1,000-bp. This large set of A-ESTs will be a valuable resource for gene expression studies and crop improvement in cultivated allotetraploid cotton.