Effect of overexpression of IPW on RNA expression in fetal RPE

To reveal the clinical significance, pathological involvement and molecular mechanism of IPW in retinal pigment epithelium anomalies that contribute to age-related macular degeneration. Microarrays were performed to detect IPW expression under pathological conditions. Overall design: RNA was extracted with its concentration and purity measured using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific), and its integrity assessed using the RNA Nano 6000 Assay Kit on the Agilent Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, USA). A total amount of 1 µg RNA was sent for sequencing library generation using the Hieff NGS Ultima Dual-mode mRNA Library Prep Kit for Illumina (Yeasen Biotechnology, Shanghai, China) following the manufacturer's recommendations. The library fragments were then purified with the AMPure XP system (Beckman Coulter), and sequenced on an Illumina NovaSeq platform to generate 150 bp paired-end reads, according to the manufacturer's instructions.