Mutants and effects of cross-link formation.
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The table shows for the six EF1-mutants MM10, GH54, FH4, GH19, PP2, and SW3 the cross-link region and position, the ATP hydrolysis activity after reduction and oxidization, and the cross-link yield. By re-reducing the oxidized samples the activity could be restored (GH54: 93%, FH4: 45%, GH19: 100%). The wild type KH7 denotes the enzyme without cysteines for cross-linking the rotor to the stator. The data for KH7, MM10, PP2, and SW3 were taken from [17].
创建时间:
2015-12-02



