Gene expression of clonal subpopulations derived from the 4T1 murine mammary carcinoma cell line

Breast tumors often exhibit intratumoral heterogeneity. We hypothesized that phenotypically distinct clonal subpopulations in heterogeneous tumors may affect overall tumor morphology and cancer cell invasion. In order to study heterogeneous tumors, we isolated individual cells from a parental 4T1 murine mammary carcinoma cell line and generated four clonal subpopulations (E1, E2, A, and M). To characterize these subpopulations, we examined gene expression by performing bulk RNA-sequencing. Overall design: We performed gene expression analysis on clonal, iRFP-expressing subpopulations derived from a parental population of 4T1 murine mammary carinoma cells. We collected RNA from three biological replicates for each sample.