Exploration of lncRNA/circRNA-miRNA-mRNA networks in patients with chronic atrophic gastritis in plateau areas [lncRNA]

Higher incidence of chronic atrophic gastritis (CAG) is generally considered a precancerous lesion of gastric cancer (GC). Therefore, the early diagnosis and treatment of CAG, especially in Tibetan Plateau areas, play an important role in the prevention of GC. The atrophic and non-atrophic gastric mucosal tissue samples from 7 patients with chronic gastritis (CG) and cancer tissue samples from 3 patients with GC were collected. High-throughput sequencing was performed to identify the differentially expressed in lncRNAs, circRNAs, miRNAs, and mRNAs, followed by the construction of competitive endogenous RNA (ceRNA) regulatory networks (lncRNA/circRNA-miRNA-mRNA network) in CAG. Those differentially expressed mRNAs with the same expression trend in both CAG and GC were further identified. Two datasets (GSE153224 and GSE163416), involving data in non-Tibetan Plateau areas, were used to further screen out plateau-specific mRNAs in CAG, followed by identification of the plateau-specific and ferroptosis related mRNAs. GO and KEGG enrichment analysis were performed to investigate the biological functions of plateau-specific mRNAs in CAG. This study may provide useful information for identifying potential biomarkers for the diagnosis of CAG. Overall design: The atrophic and non-atrophic gastric mucosal tissue samples from 7 patients with chronic gastritis (CG) and cancer tissue samples from 3 patients with GC were collected. High-throughput sequencing was performed to identify the differentially expressed in lncRNAs, circRNAs, miRNAs, and mRNAs, followed by the construction of competitive endogenous RNA (ceRNA) regulatory networks (lncRNA/circRNA-miRNA-mRNA network) in CAG.