Endometrial decidualization status modulates endometrial microvascular complexity and trophoblast outgrowth in gelatin methacryloyl hydrogels

The endometrium undergoes rapid cycles of vascular growth, remodeling, and breakdown during the menstrual cycle and pregnancy. Decidualization is an endometrial differentiation process driven by steroidal sex hormones that is critical for blastocyst-uterine interfacing and blastocyst implantation. Certain pregnancy disorders may be linked to decidualization processes. However, much remains unknown regarding the role of decidualization and reciprocal trophoblast-endometrial interactions on endometrial angiogenesis and trophoblast invasion. Here, we report an engineered endometrial microvascular network embedded in gelatin methacryloyl hydrogels that displays morphological and functional patterns of decidualization. We show vessel complexity and soluble factor secretion are sensitive to decidualization and affect trophoblast motility. We also show that in our system, the signaling between endometrial and trophoblast cells was not bi-directional. Although endometrial microvascular network ..., Please see the linked manuscript for the most detailed methods section. The data were collected at the University of Illinois Urbana-Champaign, processed using a personal laptop computer (Mac) if necessary, and subsequently included in the manuscript., , # Data from: Endometrial decidualization status modulates endometrial microvascular complexity and trophoblast outgrowth in gelatin methacryloyl hydrogels

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Data included are raw and binarized image files. MATLAB code for the image analysis is uploaded as well.

## Description of the Data and file structure

Notation of title name includes Date_Condition1_Condition2_hydrogelnumber_ROInumber_WavelengthImaged.Â