RNA-seq of HUVECs stimulated with HG and oxLDL

We investigated the role of SNO-GNIA2 in HG+oxLDL-induced endothelial inflammation during the development of diabetes-accelerated atherosclerosis and found that SNO-GNAI2 could promote endothelial inflammation through dysregulating Hippo-YAP . We hypothesized that SNO-GNAI2 induced Hippo-YAP dysfunction through enhancing coupling and activating G-protein coupling receptors (GPCRs). We determined the role of SNO-GNIA2 in HG+oxLDL induced endothelial inflammation during the development of diabetes-accelerated atherosclerosis and tried to investigate the underlying mechanism, mRNA profiles of HUVECs stimulate with HG andoxLDL were generated by deep sequencing, in duplicate, using Illumina HiSeq. The sequence reads that passed quality filters were analyzed at the transcript isoform level. Fragments Per Kilobase per Million bases (FPKM) analysis was used to evaluate the level of each gene.