Transcriptome profiling of rat brain samples in two age groups in responding to intracerebral hemorrhage. Transcriptome profiling of rat brain samples in two age groups in responding to intracerebral hemorrhage

Aging-induced decline of endogenous neuroprotection has been shown to aggravate intracerebral hemorrhage (ICH)-induced acute brain injury, however, the underlying mechanisms in brain are still little known. In this study, we applied a rat ICH model to study the transcriptional responses in the early and late aging (13-month and 22-month old) rats that show substantial differences in brain damage and recovery. Transcriptome analysis (RNA-seq) reveals that brain expression of neuroinflammation genes is similarly and selectively upregulated in ICH, which includes genes in the cellular response to interferon gamma function. We show that the anti-IFN-γ treatment effectively reduces ICH-induced acute brain injury. Overall design: Rats from two age groups, including 13-month and 22-month, were selected to perform experiments. Left brains of rats were treated with intracerebral hemorrhage, while right brains were as control. Three biological replicates were used to perform RNA-seq experiments. A total of 12 RNA-seq samples were obtained.