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ChIP-seq analysis of RpoS in Vibrio cholerae

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NIAID Data Ecosystem2026-05-10 收录
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https://www.ncbi.nlm.nih.gov/sra/ERP163974
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The experiment contains ChIP-seq data for an rpoS- version of Vibrio cholerae strain A1552, or a derivative encoding rpoS-3xFLAG. In both cases, smooth colony variants were used. The strains were both grown at 37 degrees, in LB medium, to an OD600 of 2.0, and crosslinked with 1 % (v/v) formaldehyde. After sonication, to break open cells and fragment DNA, immunoprecipitations were done using anti-FLAG antibodies. Libraries were prepared using DNA remaining after immunoprecipitation.
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2026-01-20
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