Comparison of PCR results.

The table lists the results of the MRSA screening with the LightCycler MRSA Advanced and the Detect-Ready MRSA test. True and false positive and negative values were determined by comparing the PCR results to the corresponding culture result. The results of the CHROMagar MRSA plates were set as gold standard. All positive cultural results were confirmed by the microbiological laboratory of our clinic. Two samples were tested positive in both PCRs and on one of the cultures and were treated as positive cultural samples for the determination of true und false positive values as well. Negative results of the Detect-Ready MRSA test are results not identified as MRSA by the test software.