Sequencing of fast and slow muscle of pacu

Fish myotomal muscle is composed of anatomically segregated fibre types (slow, intermediate and fast) with distinct metabolic phenotypes. Pacu (Piaractus mesopotamicus) is a member of the Characiform family native to the Prata Basin (South America) and is a target of aquaculture industry. The main limitations for the development of a selective breeding program for this species are the lack of genetic information. The primary objectives of the present study were 1) to use NGS to increase the genetic resources available for Pacu, 2) to exploit the anatomical separation of fibre types to compare the transcriptomes of Slow and Fast muscles. We sequenced fast and slow skeletal muscle (n=5) using a HiSeq 2000.