Schematic representation and basic characteristics of BST-2 variants.
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(A) Deletion mutagenesis of BST-2 N-terminus. The CT domain of BST-2 (red curved line under the cell membrane), fused HA peptide (purple), Myc peptide (green), Flag (cyan) and 6×His peptide (black) are represented. The constructs in the dashed box are specially designed for the nickel affinity purification and which expression test were shown in Figure 3. (B) SDS-PAGE analysis of BST-2 variants under denaturing conditions. 293T cells were transfected with 500 ng of VR1012, BST-2 WT NHA, BST-2 WT IHA, BST-2 WT NT, BST-2 ΔN20 NHA, BST-2 ΔN20 IHA, BST-2 ΔN20 NT, BST-2 ΔN20 NMyc, BST-2 ΔN20 NFlag or BST-2 ΔTM 159H6. After 48 h, cells were harvested and analyzed by Western blotting using an anti-BST-2 pAb, anti-HA mAb, anti-myc mAb, anti-flag mAb and anti-tubulin mAb, respectively. The major bands corresponding to higher and lower glycosylated BST-2 are marked with symbols.
创建时间：
2014-10-27
