Metabolic Output of Human Primary Preadipocytes.

Metabolic parameters of human primary adipocytes infected with Ad-ADAM12S (n = 10) or Ad-MOCK (n = 10) were assessed using the Seahorse XF24 analyzer under basal conditions, following catecholamine exposure, and after treatment with a stressor mix of FCCP and oligomycin. Extracellular acidification rate (ECAR) was measured as an indicator of enhanced glycolytic activity in ADAM 12S expressing preadipocytes compared to mock infected preadipocytes. * indicates comparisons between the basal and stressed states of ADAM12S-infected cells. ** denotes comparisons between ADAM 12S- and mock-infected cells for each condition, including the stressor mix and catecholamine treatments (p < 0.05).