Effect of EGF receptor antagonists or HB-EGF neutralizing antibody on chemotactic migration of St-T1b cells or hESCs.

(A) Decidualized St-T1b cells were preincubated in transwell migration inserts with the tyrosine kinase inhibitor AG1478 (100 nM) or the EGFR1/2/4 (HER1/HER2/HER4) inhibitor (10 nM) for 1 h before addition of HB-EGF or TCM to the lower compartment for another 18 h. The percentage of migrated cells relative to the total cell number is given as the motility index (means±SD, n = 3). Results were analyzed by ANOVA and Dunnett or Tukey test. ***, P<0.001 compared to the respective control without inhibitor; a, P<0.001, b, P<0.01 compared to non-stimulated cells without inhibitor (open column). (B) Decidualized hESCs were subjected to transwell migration assay with PDGF-BB, HB-EGF or TCM as the chemoattractant, in the absence or presence of a neutralizing antibody to HB-EGF. Motility indices (means±SD, n = 3) were analyzed by ANOVA and Tukey test. a, P<0.001 compared to the control without chemoattractant or antibody (white column); ***, P<0.001 compared between conditions without or with antibody.