The genomic landscape of metastasis in treatment-naïve breast cancer models

Exome and whole genome sequencing of matched primary tumor and lung metastases was used to identify metastasis-specific genomic events that drive metastasis. Primary and metastastic tumors from PyMT and Her2 genetically engineered mouse models were isolated and sequenced. Recurrent metastasis-specific copy number alterations, single nucleotide variants, and translocations within codiing regions were assessed. The metastatic driving potential of metastasis-specific single nucleotide variants was then tested using allograft models.