A single-cell level comparison of human inner ear organoids with the human cochlea and vestibular organs

In this study, we generated a human inner ear atlas containing three stages of inner ear development. This atlas was used to evaluate the differentiation approach of human pluripotent stem cells in to complex inner ear tissue, known as inner ear organoids. The primary goal of this single-nucleus RNA-sequencing analysis was to capture the cell type diversity of the human inner ear at different stages of development. The secondary goal was to define the similarity of organoid-derived inner ear cell types with the atlas-derived human inner ear cell types and to determine the developmental stage of the organoid-derived inner ear cell types. Overall design: Single-nucleus RNA-sequencing data from two human fetal inner ears (fetal age weeks 7.5 and 9.2) and one human adult inner ear.