Gene expression profiles of MLL-AF9 leukemia cells in C57BL/6 mice and Rag2-/- mice

The relative importance of T/B lymphocytes and NK cells during acute myeloid leukemia (AML) progression has been unclear. In this study, we compared gene expression profiles of mouse AML cells expressing MLL-AF9 collected from immunologically normal wild-type (WT) C57BL/6 mice, Rag2-/- mice lacking mature T and B cells, and NSG mice lacking T, B and NK cells. Overall design: MLL-AF9 leukemia cells coexpressing GFP were transplanted into immunologically normal C57BL/6 mice, Rag2-/- mice lacking mature T and B cells, and NSG mice lacking T, B and NK cells. GFP+ leukemia cells were collected from bone marrow and isolated using cell sorter before RNA extraction.