Florigen family chromatin recruitment, competition and targets [RNA-Seq]

This study identified genes that re-upregulated in far-red enriched photoperiod in the wild type or tfl1-1, but not in ft-10 (FT-dependent photoinduction). We performed RNA seq on tfl1-1, wild type and ft-10 grown for 42 days in short-day conditions and treated for 24 hours with far-red enriched photoinduction (24 hr FRP) or grown for 43 days in short-day conditions (no FRP). RNA was isolated from trimmed and dissected non-bolted inflorescences. Overall design: We sequenced three biological replicates per genotype and treatment and analyzed two biological replicates per condition for differential expression.