Comprehensive analysis of transcriptome-wide m⁶A methylome in the anterior capsule of the lens of high myopia patients

To assess the m⁶A methylome in the anterior capsule of the lens of high myopia patients. MeRIP-seq and RNA-seq were performed to identify differences in the m⁶A methylomes and gene expression between anterior capsule of the lens of simple nuclear cataract patients (N) and nuclear cataract patients with high myopia (G). Expression of m⁶A-related enzymes was confirmed by quantitative real-time-PCR. ALKBH5 was downregulated in G. The observed m⁶A peak was identical to the conserved RRACH gmotif and was markedly correlated with two distinct coordinates. Differentially methylated genes were enriched in some pathways regulating the formation of extracellular matrix. These findings suggest that upregulation of m⁶A methylation may change fundus anatomy by regulating the composition of the extracellular matrix through encoding protein.