SBF-SEM from a patient derived tissue biopsies (diabetic eye)
收藏DataCite Commons2024-10-16 更新2025-04-16 收录
下载链接：
https://etsin.fairdata.fi/dataset/cd102ffe-f5b6-46fb-8fb8-e5df3b2ecdad
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资源简介：
Immediately after surgical excision, the fibrovascular tissue was fixed with 2.5% GA, 2% FA, 2mM CaCl2 in 0.1 M Na-cacodylate buffer, pH 7.4 for 2 hours at room temperature. After fixation, the fixative was replaced with fixative including 2% FA in the same buffer prior to preparation of the specimen for SBF-SEM with double osmication. Image series were
acquired with FEG-SEM Quanta 250 equipped with a microtome, using a backscattered electron detector. The imaging was done using 0.3-Torr pressure, 2.5 kV beam voltage and spot size 3. Image processing, segmentation and visualization were done using MIB and Amira.
提供机构：
University of Helsinki
创建时间：
2024-10-16
