Comparative study of mRNAs and lncRNAs in endothelial and non-endothelial cells

To identify lncRNAs enriched in ECs, a microarray was performed to profile ~30,000 lncRNAs and ~26,000 coding transcripts using an Arraystar human LncRNA microarray v3.0 system (Arraystar, Rockville, MD). Three primary human EC lines and two non-EC lines at low passages, namely, human umbilical vein EC (HUVEC), human retinal EC (HREC), human choroidal EC, human dermal fibroblast cell (HDF) and human retinal pigment epithelial (RPE) cell lines, were used in the array. Purity of EC lines was confirmed by acetyl-LDL uptake and EC marker staining The expression of long noncoding RNAs and mRNAs was studied using Arraystar human LncRNA microarray v3.0 system using five cell types: human umbilical vein endothelial cells (HUVEC), human retinal endothelial cells (HREC), human choroidal endothelial cells (HCEC), human dermal fibrsolast cells (HDEF) and ARPE-19 cells. The goal of the design is to find endothelial specific lncRNAs and mRNAs.