Gene expression changes in mouse kidney between 4 and 24-26 months of age

Expression of many genes change as a function of age, but the specific genes and extent of expression changes differs between different conditions. In this work we perform whole genome sequencing on RNA extracted from the kindeys of male and female wildtype mice at 4 and 24-26 months of age to assess changes in gene expression and alterations in pathways related to aging. To assess the effect of age on gene expression in the kidney mice were euthanized at 4 or 24-26 months of age under fed conditions and kidney tissue was snap frozen in liquid nitrogen. We then performed gene expression profiling using RNAseq data obtained from these tissues.