Secondary peptide ratio estimation.

Here the Gaussian mixture method is used to recover the peptide ratio of the second peptide in a convolved set. The error in the estimation of an Ang-(1–9) peak ratio against its corresponding SIS peptide when convolved with various amounts of Ang-(2–10) and its corresponding SIS is used to test the Gaussian mixture method. The initial concentration of 300 nM of each peptide (for a 1∶1∶1∶1 ratio of peptides, Material S2) is modified by changing the amount of Ang-(2–10). Here we see that the Gaussian mixture method can recover the second peptide from a series of different peptide ratios.Secondary peptide ratio estimation.