An siRMSD parameter of structural distortion induced by chemical modification is predictive of the off-target effect of siRNA_(1/2)

To investigate effects of siRNA with various 2'-ribose modifications on genome-wide off-target effect, we analyzed gene expression profile of HeLa cells transfected by their siRNAs. Total RNAs were extracted from HeLa cells transfected by mock, unmodified, 2'-formamido, 2'-deoxy, or 2'-O-methoxyethyl modified siRNA and performed microarray analysis.