Identification of Hepatic Genes Regulated by ATF3 using Next Generation Sequencing. Identification of Hepatic Genes Regulated by ATF3 using Next Generation Sequencing

This study aimed to investigate how many genes were regulated by hepatocyte ATF3. C57BL/6J mice were injected intravenously with adeno-associated virus (AAV) expressing human activating transcription factor 3 (ATF3) under the control of an albumin promoter (AAV8-ALB-ATF3) or AAV8-ALB-Null). After 8 weeks, liver samples were used for RNA sequencing using Illumina NextSeq 500. Over 3000 genes were found to be up- or down-regulated by ATF3. Overall design: Ten weeks old C57BL/6J mice were intravenoulsy injected with AAV8-ALB-ATF3 or AAV8-ALB-Null (control) (n=8 per group). After 8 weeks, total RNA was extracted and cDNA libraries were prepared. RNA sequencing was carried out using Next-Seq 500. Data were analyzed using Illumina Softwares in BaseSpace.