Nanopore sequencing of RNA and cDNA molecules expands the transcriptomic toolbox in prokaryotes

In this study, we applied and benchmarked all currently available ONT library preparation methods to analyse RNAs in the prokaryotic model organism Escherichia coli K-12. These include direct sequencing of native RNAs, sequencing of native cDNAs, and sequencing of PCR-amplified cDNAs. Here, you find the basecalled and demultiplexed FASTQs and minimap2-mapped BAM files from untrimmed reads.