Toehold-Mediated Nonenzymatic DNA Strand Displacement analysis dataset

This dataset comprises the results of analysis of toehold-mediated DNA strand displacement. The results demonstrate a new, simpler approach for the analysis of real-life DNA samples using a toehold-mediated DNA strand displacement reaction where a partially complementary duplex, with a ss-toehold sequence, was used as a target for analysis.The approach was successfully implemented for human DNA genotyping for gender identification and it is envisaged that this model system could be easily adapted to other DNA genotyping molecular diagnostics.Human genomic and mitochondrial DNA were coextracted from the author’s own blood samples. The analysis of this DNA consisted of two stages:(i) PCR amplification was used to generate a PCR product with a contiguous ss-toehold domain; and(ii) toehold-mediated DNA strand displacement reactions between the target toehold-PCR product and single-stranded chemically synthesized oligonucleotide molecular probes were performed using a quenched Föster resonance energy transfer (FRET) technique to monitor how the reaction proceeded.Copyright (c) 2013 Flinders University, South AustraliaDate coverage: 2012-01-01 - 2012-06-01

本数据集包含了基于踮脚介导的DNA链位移分析的结果。该研究展示了利用踮脚介导的DNA链位移反应分析现实生活DNA样本的一种新颖、简便的方法。在此方法中，部分互补的双链DNA，带有单链踮脚序列，被用作分析的目标。该方法已成功应用于人类DNA基因分型以进行性别鉴定，且预期该模型系统可轻易地适应其他DNA基因分型分子诊断。作者从自身血液样本中共同提取了人类基因组DNA和线粒体DNA。该DNA分析分为两个阶段：(i) 利用PCR扩增技术生成具有连续单链踮脚域的PCR产物；(ii) 通过采用淬灭的福斯特共振能量转移（FRET）技术，对目标踮脚PCR产物与单链化学合成的寡核苷酸分子探针之间的踮脚介导的DNA链位移反应进行监测，以追踪反应进程。版权所有（c）2013弗林德斯大学，澳大利亚。数据覆盖时间：2012年1月1日至2012年6月1日。